4 January 2008 Micro amperometric immunosensor by antibody immobilizing with electropolymerized protein A
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Abstract
A micro plane amperometric immunosensor for the detection of human immunoglobulin G is fabricated on silicon wafer based on Micro-Electro-Mechanical Systems (MEMS) technology. This microsensor is an electrochemical system composed of two-electrode (working electrode and counter electrode) integrated with a micro reaction cell made of SU-8 photoresist. The sensitive area of the working electrode is only 1 mm2. A new method for the orientation-controlled immobilization of antibody based on staphylococcal protein A (SPA) and polypyrrole (PPy) is developed, which is fast, controllable and proper to microelectrode. PPy as a transition layer is first electropolymerized at the sensing area of the working electrode. Then SPA is co-electropolymerized with PPy for further orientedy immobilization of antibody. The electrodes modified by PPy and the coelectropolymer of PPy and SPA were characterized by SEM. HIgG range from 5 ng/ml to 640 ng/ml can be detected by this immunosensor with less consumption of reagent and acceptable reproducibility and stability.
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Chao Bian, Jizhou Sun, Lan Qu, Shanhong Xia, "Micro amperometric immunosensor by antibody immobilizing with electropolymerized protein A", Proc. SPIE 6836, MEMS/MOEMS Technologies and Applications III, 68360P (4 January 2008); doi: 10.1117/12.775058; https://doi.org/10.1117/12.775058
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