8 February 2008 Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo
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Proceedings Volume 6842, Photonic Therapeutics and Diagnostics IV; 684205 (2008); doi: 10.1117/12.762937
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
Multiphoton optical tomography provides non-invasive optical sectioning of skin with high spatial and intracellular resolution. The imaging system DermaInspect based on femtosecond laser pulses and equipped with a SPC 830 card for FLIM analysis was used to perform this technique in vivo in clinical studies on patients with abnormal pigmented tissues. After examination in vivo, biopsies were taken and analyzed again. So far it was possible to distinguish between normal and diseased tissues mainly due to their different morphological appearances in the imaging mode: melanoma tissues show differences in the structure of the epidermal layers and the presence of dendritic cells compared to normal skin. The optical analysis can be improved when using the system in the time-correlated single photon counting mode. Fluorescence lifetime analysis reveals characteristic differences between healthy and melanoma tissues even before the visible morphological changes in human tissue in vivo.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Iris Riemann, Alexander Ehlers, Ronan Le Harzic, Enrico Dimitrow, Martin Kaatz, P. Elsner, Rainer Bückle, Karsten König, "Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo", Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684205 (8 February 2008); doi: 10.1117/12.762937; https://doi.org/10.1117/12.762937
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KEYWORDS
Tissues

Melanoma

Skin

Fluorescence lifetime imaging

In vivo imaging

Biopsy

Proteins

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