Optical mechanisms for detection of lipid-rich atherosclerotic plaques by near-infrared spectroscopy

Edward L. Hull; Craig M. Gardner; James E. Muller M.D.; Vianna J. Muller; Christopher V. Salvato; Jennifer B. Lisauskas; Jay D. Caplan

doi:10.1117/12.761723

8 February 2008 Optical mechanisms for detection of lipid-rich atherosclerotic plaques by near-infrared spectroscopy

Edward L. Hull, Craig M. Gardner, James E. Muller M.D., Vianna J. Muller, Christopher V. Salvato, Jennifer B. Lisauskas, Jay D. Caplan

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Proceedings Volume 6842, Photonic Therapeutics and Diagnostics IV; 68422A (2008) https://doi.org/10.1117/12.761723
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

InfraReDx has developed a spectroscopic cardiac catheter system capable of acquiring near-infrared (NIR) reflectance spectra from coronary arteries in vivo for identification of lipid-rich plaques of interest (LRP). The spectral data are analyzed with a chemometric model, producing a hyperspectral image (a chemogram) used to identify LRP in the interrogated region. In this paper, we describe a FT-IR microscopy system for measurement of the NIR scattering and absorption properties of healthy and diseased regions of human coronary arteries in small volumes (~10 μl). Scattering and absorption coefficients are obtained from sequential 140 um x 140 um regions of interest across the face of 500-micron thick, saline-irrigated fresh coronary artery sections. A customized FTIR microscope, measurement protocol, and inversion algorithm are used for optical property determination, and the system is calibrated using measurements of tissue-simulating phantoms having well-characterized optical properties. Tissue optical properties are co-registered with brightfield transmission images as well as with stained histologic thin sections (H&E, Movat Pentachrome, and Oil Red O) acquired from an immediately-adjacent section. The ultimate goal of these experiments is to establish a mechanistic link between the multivariate model predictions displayed on the InfraReDx chemogram and the light-tissue interactions that govern the measured NIR reflectance spectra.

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Edward L. Hull, Craig M. Gardner, James E. Muller M.D., Vianna J. Muller, Christopher V. Salvato, Jennifer B. Lisauskas, and Jay D. Caplan "Optical mechanisms for detection of lipid-rich atherosclerotic plaques by near-infrared spectroscopy", Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422A (8 February 2008); https://doi.org/10.1117/12.761723

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KEYWORDS

Optical properties

Calibration

Data modeling

Scattering

Arteries

Absorption

Near infrared

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