Role of superoxide dismutase in the photochemical response of cultured RPE cells to laser exposure at 413 nm

Michael L. Denton; Michael S. Foltz; Kurt J. Schuster; Larry E. Estlack; Robert J. Thomas

doi:10.1117/12.763340

25 February 2008 Role of superoxide dismutase in the photochemical response of cultured RPE cells to laser exposure at 413 nm

Michael L. Denton, Michael S. Foltz, Kurt J. Schuster, Larry E. Estlack, Robert J. Thomas

Author Affiliations +

Proceedings Volume 6844, Ophthalmic Technologies XVIII; 68441O (2008) https://doi.org/10.1117/12.763340
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Thresholds for photochemical damage were performed in RPE cell lines (artificially pigmented) taken from either human (hTERT-RPE1), wild type (wt) mouse, or transgenic mice deficient (+/-) in either superoxide dismutase 1 (SOD1) or SOD2. The four cell lines were characterized by immunohistochemical and immunoblot analyses to determine relative abundance of the SOD proteins. There was no difference in sensitivity between the human, murine wt and murine SOD1-deficient cells, whereas there was a dramatic (2 fold) increase in threshold irradiance value for the murine SOD2-deficient cells. Possible explanations for the unexpected result are provided.

Citation Download Citation

Michael L. Denton, Michael S. Foltz, Kurt J. Schuster, Larry E. Estlack, and Robert J. Thomas "Role of superoxide dismutase in the photochemical response of cultured RPE cells to laser exposure at 413 nm", Proc. SPIE 6844, Ophthalmic Technologies XVIII, 68441O (25 February 2008); https://doi.org/10.1117/12.763340

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