25 February 2008 Role of superoxide dismutase in the photochemical response of cultured RPE cells to laser exposure at 413 nm
Author Affiliations +
Proceedings Volume 6844, Ophthalmic Technologies XVIII; 68441O (2008) https://doi.org/10.1117/12.763340
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
Thresholds for photochemical damage were performed in RPE cell lines (artificially pigmented) taken from either human (hTERT-RPE1), wild type (wt) mouse, or transgenic mice deficient (+/-) in either superoxide dismutase 1 (SOD1) or SOD2. The four cell lines were characterized by immunohistochemical and immunoblot analyses to determine relative abundance of the SOD proteins. There was no difference in sensitivity between the human, murine wt and murine SOD1-deficient cells, whereas there was a dramatic (2 fold) increase in threshold irradiance value for the murine SOD2-deficient cells. Possible explanations for the unexpected result are provided.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael L. Denton, Michael L. Denton, Michael S. Foltz, Michael S. Foltz, Kurt J. Schuster, Kurt J. Schuster, Larry E. Estlack, Larry E. Estlack, Robert J. Thomas, Robert J. Thomas, } "Role of superoxide dismutase in the photochemical response of cultured RPE cells to laser exposure at 413 nm", Proc. SPIE 6844, Ophthalmic Technologies XVIII, 68441O (25 February 2008); doi: 10.1117/12.763340; https://doi.org/10.1117/12.763340
PROCEEDINGS
8 PAGES


SHARE
Back to Top