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11 February 2008 Time-resolved luminescence measurements of the magnetic field effect on paramagnetic photosensitizers in photodynamic reactions
O. Mermut, J.-P. Bouchard, J.-F. Cormier, P. Desroches, K. R. Diamond, M. Fortin, P. Gallant, S. Leclair, J.-S. Marois, I. Noiseux, J.-F. Morin, M. S. Patterson, M. Vernon
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Proceedings Volume 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII; 68450T (2008) https://doi.org/10.1117/12.763461
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
The development of multimodal molecular probes and photosensitizing agents for use in photodynamic therapy (PDT) is vital for optimizing and monitoring cytotoxic responses. We propose a combinatorial approach utilizing photosensitizing molecules that are both paramagnetic and luminescent with multimodal functionality to perturb, control, and monitor molecular-scale reaction pathways in PDT. To this end, a time-domain single photon counting lifetime apparatus with a 400 nm excitation source has been developed and integrated with a variable low field magnet (0- 350mT). The luminescence lifetime decay function was measured in the presence of a sweeping magnetic field for a custom designed photosensitizing molecule in which photoinduced electron transfer was studied The photosensitizer studied was a donor-acceptor complex synthesized using a porphyrin linked to a fullerene molecule. The magneto-optic properties were investigated for the free-base photosensitizer complex as well as those containing either diamagnetic (paired electron) or paramagnetic (unpaired electron) metal centers, Zn(II) and Cu(II). The magnetic field was employed to affect and modify the spin states of radical pairs of the photosensitizing agents via magnetically induced hyperfine and Zeeman effects. Since the Type 1 reaction pathway of an excited triplet state photosensitizer involves the production of radical species, lifetime measurements were conducted at low dissolved oxygen concentration (0.01ppm) to elucidate the dependence of the magnetic perturbation on the photosensitization mechanistic pathway. To optimize the magnetic response, a solvent study was performed examining the dependence of the emission properties on the magnetic field in solutions of varying dielectric constants. Lastly, the cytotoxicity in murine tumor cell suspensions was investigated for the novel porphyrin-fullerene complex by inducing photodynamic treatments and determining the associated cell survival.
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O. Mermut, O. Mermut, J.-P. Bouchard, J.-P. Bouchard, J.-F. Cormier, J.-F. Cormier, P. Desroches, P. Desroches, K. R. Diamond, K. R. Diamond, M. Fortin, M. Fortin, P. Gallant, P. Gallant, S. Leclair, S. Leclair, J.-S. Marois, J.-S. Marois, I. Noiseux, I. Noiseux, J.-F. Morin, J.-F. Morin, M. S. Patterson, M. S. Patterson, M. Vernon, M. Vernon, "Time-resolved luminescence measurements of the magnetic field effect on paramagnetic photosensitizers in photodynamic reactions", Proc. SPIE 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII, 68450T (11 February 2008); doi: 10.1117/12.763461; https://doi.org/10.1117/12.763461
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