18 February 2008 Versatile confocal/optical coherence tomography system for embryonic developmental imaging
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Abstract
An Optical Coherence Tomography (OCT) imaging system has been designed and constructed to acquire images of scattering biological samples. By simultaneously acquiring and displaying high resolution en-face (C-scan) OCT and Laser Scanning Confocal images of Drosophila melanogaster embryos we demonstrate the potential of the system to be used as a powerful tool for imaging in Drosophila embryonic development. The system can equally be used for non invasive visualizations and measurements of the movement of Drosophila melanogaster larval heart and can easily be switched in the OCT B-scan regime. The confocal channel adds guidance as the specimen can be quickly located and this makes the use of the system in a large scale gene screen feasible.
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A. Bradu, Lisha Ma, J. Bloor, A. Podoleanu, "Versatile confocal/optical coherence tomography system for embryonic developmental imaging", Proc. SPIE 6847, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XII, 68471W (18 February 2008); doi: 10.1117/12.765824; https://doi.org/10.1117/12.765824
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