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25 February 2008 Clinical instrument for spectral diagnosis of cutaneous malignancy
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We present a novel probe-based portable clinical system for early detection and margin demarcation of melanoma and non-melanoma skin cancers. The system collects both white light reflectance and fluorescence from tissue in real time. We use gated detection to eliminate effects of room lights and make the system clinically compatible. Instrument control and spectral calibration is automated using a personal computer. The total acquisition time for data collection is less than a second. We use a spectrally-constrained inverse model for our probe geometry to fit the diffuse reflectance and extract hemoglobin content, oxygen saturation, tissue micro-architecture and melanin content. We demonstrate system performance and present results from tissue simulating phantoms. The mean rms errors in estimating scattering and absorption coefficients in tissue phantoms over a physiologically relevant range were 9.8% and 11.8% respectively. Using a photon migration model and least-squares regression we were able to extract the intrinsic fluorescence line shapes and estimate fluorophore concentrations from measured fluorescence spectra with an rms error of less than 10%.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Narasimhan Rajaram, Timothy J. Aramil, Kelvin Lee, and James W. Tunnell "Clinical instrument for spectral diagnosis of cutaneous malignancy", Proc. SPIE 6848, Advanced Biomedical and Clinical Diagnostic Systems VI, 68480R (25 February 2008);

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