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6 March 2008 Validation of a fiber optic-based UVA-VIS optical property measurement system
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Tissue optical properties at ultraviolet A (UVA) and visible (VIS) wavelengths are needed to elucidate light-tissue interaction effects and optimize design parameters for spectroscopy-based neoplasia detection devices. Toward the goal of accurate and useful in vivo measurements, we have constructed and evaluated a system for optical property measurement at UVA-VIS wavelengths. Our approach involves a neural network-based inverse model calibrated with reflectance datasets simulated using a condensed Monte Carlo approach with absorption coefficients as high as 80 cm-1 and reduced scattering coefficients as high as 70 cm-1. Optical properties can be predicted with the inverse model based on spatially resolved reflectance measured with a fiberoptic probe. Theoretical evaluation of the inverse model was performed using simulated reflectance distributions at random optical properties. Experimental evaluation involved the use of tissue phantoms constructed from bovine hemoglobin and polystyrene microspheres. An average accuracy of ±1.0 cm-1 for absorption coefficients and ±2.7 cm-1 for reduced scattering coefficients was found from realistic phantoms at five UVA-VIS wavelengths. While accounting for the very high attenuation levels near the 415 nm Soret absorption band required some modifications, our findings provide evidence that the current approach produces useful data over a wide range of optical properties, and should be particularly useful for in vivo characterization of highly attenuating biological tissues.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Quanzeng Wang, Anant Agrawal, Nam Sun Wang, and Josh Pfefer "Validation of a fiber optic-based UVA-VIS optical property measurement system", Proc. SPIE 6849, Design and Quality for Biomedical Technologies, 68490G (6 March 2008);

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