Validation of luminescent source reconstruction using spectrally resolved bioluminescence images

John M. Virostko; Alvin C. Powers; E. Duco Jansen

doi:10.1117/12.771416

12 February 2008 Validation of luminescent source reconstruction using spectrally resolved bioluminescence images

John M. Virostko, Alvin C. Powers, E. Duco Jansen

Proceedings Volume 6849, Design and Quality for Biomedical Technologies; 68490I (2008) https://doi.org/10.1117/12.771416
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

This study examines the accuracy of the Living Image® Software 3D Analysis Package (Xenogen, Alameda, CA) in reconstruction of light source depth and intensity. Constant intensity light sources were placed in an optically homogeneous medium (chicken breast). Spectrally filtered images were taken at 560, 580, 600, 620, 640, and 660 nanometers. The Living Image® Software 3D Analysis Package was employed to reconstruct source depth and intensity using these spectrally filtered images. For sources shallower than the mean free path of light there was proportionally higher inaccuracy in reconstruction. For sources deeper than the mean free path, the average error in depth and intensity reconstruction was less than 4% and 12%, respectively. The ability to distinguish multiple sources decreased with increasing source depth and typically required a spatial separation of twice the depth. The constant intensity light sources were also implanted in mice to examine the effect of optical inhomogeneity. The reconstruction accuracy suffered in inhomogeneous tissue with accuracy influenced by the choice of optical properties used in reconstruction.

Citation Download Citation

John M. Virostko, Alvin C. Powers, and E. Duco Jansen "Validation of luminescent source reconstruction using spectrally resolved bioluminescence images", Proc. SPIE 6849, Design and Quality for Biomedical Technologies, 68490I (12 February 2008); https://doi.org/10.1117/12.771416

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