12 February 2008 Fluorescence lifetime estimation of multiple near-infrared dyes in mice
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Abstract
In order to precisely recover fluorescence lifetimes from bulk tissues, one needs to employ complex light propagation models (e.g., the radiative transfer equation or a simpler yet consistent approximation, the diffusion equation) requiring knowledge of the tissue optical properties. This can be computationally expensive and therefore not practical in many applications. We present a novel method to estimate the fluorescence lifetimes of multiple fluorophores embedded in mice. By assuming that the photon diffusion does not significantly change the fluorescence decay slope, the light propagation is simply modeled as a time-delay during lifetime estimation. Applications of this approach are demonstrated by simulation, phantom data, and in vivo experiments.
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Guobin Ma, Guobin Ma, Simon Fortier, Simon Fortier, Muriel Jean-Jacques, Muriel Jean-Jacques, Niculae Mincu, Niculae Mincu, Frederic Leblond, Frederic Leblond, Zahia Ichalalene, Zahia Ichalalene, Anader Benyamin-Seeyar, Anader Benyamin-Seeyar, Mario Khayat, Mario Khayat, } "Fluorescence lifetime estimation of multiple near-infrared dyes in mice", Proc. SPIE 6850, Multimodal Biomedical Imaging III, 685003 (12 February 2008); doi: 10.1117/12.763544; https://doi.org/10.1117/12.763544
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