14 February 2008 Hyperspectral small animal fluorescence imaging: spectral selection imaging
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Abstract
Molecular imaging is a rapidly growing area of research, fueled by needs in pharmaceutical drug-development for methods for high-throughput screening, pre-clinical and clinical screening for visualizing tumor growth and drug targeting, and a growing number of applications in the molecular biology fields. Small animal fluorescence imaging employs fluorescent probes to target molecular events in vivo, with a large number of molecular targeting probes readily available. The ease at which new targeting compounds can be developed, the short acquisition times, and the low cost (compared to microCT, MRI, or PET) makes fluorescence imaging attractive. However, small animal fluorescence imaging suffers from high optical scattering, absorption, and autofluorescence. Much of these problems can be overcome through multispectral imaging techniques, which collect images at different fluorescence emission wavelengths, followed by analysis, classification, and spectral deconvolution methods to isolate signals from fluorescence emission. We present an alternative to the current method, using hyperspectral excitation scanning (spectral selection imaging), a technique that allows excitation at any wavelength in the visible and near-infrared wavelength range. In many cases, excitation imaging may be more effective at identifying specific fluorescence signals because of the higher complexity of the fluorophore excitation spectrum. Because the excitation is filtered and not the emission, the resolution limit and image shift imposed by acousto-optic tunable filters have no effect on imager performance. We will discuss design of the imager, optimizing the imager for use in small animal fluorescence imaging, and application of spectral analysis and classification methods for identifying specific fluorescence signals.
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Silas Leavesley, Silas Leavesley, Yanan Jiang, Yanan Jiang, Valery Patsekin, Valery Patsekin, Heidi Hall, Heidi Hall, Douglas Vizard, Douglas Vizard, J. Paul Robinson, J. Paul Robinson, } "Hyperspectral small animal fluorescence imaging: spectral selection imaging", Proc. SPIE 6850, Multimodal Biomedical Imaging III, 68500X (14 February 2008); doi: 10.1117/12.763935; https://doi.org/10.1117/12.763935
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