21 February 2008 Two-photon imaging using a flexible endoscope
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Proceedings Volume 6851, Endoscopic Microscopy III; 68510B (2008) https://doi.org/10.1117/12.762970
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
Two-photon autofluorescence imaging offers the analysis of cells and tissues without the need of taking biopsies, staining and complicated confocal detection systems. Therefore, it is of special interest for non- or minimal invasive clinical diagnostics. Until now, two-photon imaging was performed only on superficial surfaces like skin or of biopsies. To extend this technique to deeper tissues or inside the body the optical properties have to be reduced to endoscopical sizes. This can be achieved by tiny GRIN-optics, based on a radial gradient in the reflective index. A newly developed GRIN-lens assembly with increased numerical aperture is of special interest which is shown by the quality of tissue constituents and cell autofluorescence images. A fiber directs the laser light to the specimen in an assembly like an endoscope. This well-characterized photonic crystal fiber supports the high laser power of the femtosecond excitation impulses without the generation of non-linearities. A sensitive PMT detector detects the fluorescence. First fluorescence images using a fiber-GRIN lens combination were taken.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Iris Riemann, Selma Schenkl, Ronan Le Harzic, Daniel Sauer, Alexander Ehlers, Bernhard Messerschmidt, Rainer Bückle, Karsten König, "Two-photon imaging using a flexible endoscope", Proc. SPIE 6851, Endoscopic Microscopy III, 68510B (21 February 2008); doi: 10.1117/12.762970; https://doi.org/10.1117/12.762970
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