Fluorescent and scattering contrast agents in a mouse model of colorectal cancer

Amy M. Winkler; Photini F. S. Rice; Timothy S. Troutman; Marina V. Backer; Joseph M. Backer; Rebekah A. Drezek; Marek Romanowski; Jennifer K. Barton

doi:10.1117/12.764043

21 February 2008 Fluorescent and scattering contrast agents in a mouse model of colorectal cancer

Amy M. Winkler, Photini F. S. Rice, Timothy S. Troutman, Marina V. Backer, Joseph M. Backer, Rebekah A. Drezek, Marek Romanowski, Jennifer K. Barton

Author Affiliations +

Proceedings Volume 6851, Endoscopic Microscopy III; 68510L (2008) https://doi.org/10.1117/12.764043
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye.

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Amy M. Winkler, Photini F. S. Rice, Timothy S. Troutman, Marina V. Backer, Joseph M. Backer, Rebekah A. Drezek, Marek Romanowski, and Jennifer K. Barton "Fluorescent and scattering contrast agents in a mouse model of colorectal cancer", Proc. SPIE 6851, Endoscopic Microscopy III, 68510L (21 February 2008); https://doi.org/10.1117/12.764043

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