7 February 2008 Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila
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Abstract
Side-polished fiber immunosensor based on surface plasmon resonance (SPR) onto self-assembled protein A layer was proposed for the detection of Legionella pneumophila. A self-assembled protein A layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and activated by N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/ N-Hydroxysuccinimide (EDC/NHS). The formation of self-assembled protein A and gold layer on side-polished surface and the binding of antibody and antigen in series were confirmed by SPR response on spectrum. The binding protein A layer can improve the sensitivity, which indirectly supports the configurations that antibody layer is immobilized on the binding protein A layer with a well-ordered orientation. The surface morphology analyses of self-assembled protein A layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein A were demonstrated by SPR dip shifts on optical spectrum analyzer. The SPR fiber immunosensor for detection of L. pneumophila was developed and the detection limit was 10 CFU/ml with the SPR dip shift in wavelength from 1070 to 1105nm. The current fabrication technique of a SPR immunosensor using optical fiber for the detection of Legionella pneumophila could be applied to construct other biosensor.
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Yu-Chia Tsao, Yu-Chia Tsao, Yi-Wen Yang, Yi-Wen Yang, Woo-Hu Tsai, Woo-Hu Tsai, Tsong-Rong Yan, Tsong-Rong Yan, } "Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila", Proc. SPIE 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII, 685206 (7 February 2008); doi: 10.1117/12.758498; https://doi.org/10.1117/12.758498
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