Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging

Frances N. Keith; Rohith K. Reddy; Rohit Bhargava

doi:10.1117/12.762468

8 February 2008 Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging

Frances N. Keith, Rohith K. Reddy, Rohit Bhargava

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 685306 (2008) https://doi.org/10.1117/12.762468
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Fourier transform infrared (FT-IR) spectroscopic imaging is an emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. We demonstrate a new concept in obtaining high fidelity data using commercial array detectors coupled to a microscope and Michelson interferometer. Next, we apply the developed technique to rapidly provide automated histopathologic information for breast cancer. Traditionally, disease diagnoses are based on optical examinations of stained tissue and involve a skilled recognition of morphological patterns of specific cell types (histopathology). Consequently, histopathologic determinations are a time consuming, subjective process with innate intra- and inter-operator variability. Utilizing endogenous molecular contrast inherent in vibrational spectra, specially designed tissue microarrays and pattern recognition of specific biochemical features, we report an integrated algorithm for automated classifications. The developed protocol is objective, statistically significant and, being compatible with current tissue processing procedures, holds potential for routine clinical diagnoses. We first demonstrate that the classification of tissue type (histology) can be accomplished in a manner that is robust and rigorous. Since data quality and classifier performance are linked, we quantify the relationship through our analysis model. Last, we demonstrate the application of the minimum noise fraction (MNF) transform to improve tissue segmentation.

Citation Download Citation

Frances N. Keith, Rohith K. Reddy, and Rohit Bhargava "Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685306 (8 February 2008); https://doi.org/10.1117/12.762468

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