Divided shifted Raman spectroscopy for carotenoid detection

S. D. Bergeson; J. B. Peatross; N. J. Eyring; J. F. Fralick; S. B. Ferguson

doi:10.1117/12.765195

8 February 2008 Divided shifted Raman spectroscopy for carotenoid detection

S. D. Bergeson, J. B. Peatross, N. J. Eyring, J. F. Fralick, S. B. Ferguson

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68530A (2008) https://doi.org/10.1117/12.765195
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We have developed a compact portable instrument for resonance Raman spectroscopy of carotenoid molecules in skin tissue. Our application focuses on the 1525 cm^-1 Raman line common to all carotenoids. We use a divided shifted Raman spectroscopy (DSRS) technique that reduces sensitivity to detector drift and error. Two wavelength-narrowed LEDs illuminate the sample, and scattered light in four different wavelength channels is measured. This multi-spectral approach has single-photon sensitivity and compares favorably with laser-based Raman measurements in terms of accuracy, repeatability, and measurement time.

Citation Download Citation

S. D. Bergeson, J. B. Peatross, N. J. Eyring, J. F. Fralick, and S. B. Ferguson "Divided shifted Raman spectroscopy for carotenoid detection", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530A (8 February 2008); https://doi.org/10.1117/12.765195

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