Polarized Raman spectroscopy: application to bone biomechanics

Mekhala Raghavan; Michael D. Morris; Nadder D. Sahar; David H. Kohn

doi:10.1117/12.763520

8 February 2008 Polarized Raman spectroscopy: application to bone biomechanics

Mekhala Raghavan, Michael D. Morris, Nadder D. Sahar, David H. Kohn

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68530W (2008) https://doi.org/10.1117/12.763520
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Raman spectroscopic studies have shown that the properties of the organic matrix and the orientation of the mineral and matrix components of bone have a large influence on its properties. We employ polarized Raman microspectroscopy to monitor the changes in the orientation of mineral crystallites during tensile loading of bovine femora in the elastic regime. We load tissue in a custom-built dynamic mechanical tester that fits on the stage of a Raman microprobe and can accept hydrated tissue specimens. Parallel and perpendicular polarization components of the Raman spectra along the long axis of the diaphysis are obtained. We propose that the orientation and structure of mineral crystallites change on deformation of bone tissue by tensile loading.

Citation Download Citation

Mekhala Raghavan, Michael D. Morris, Nadder D. Sahar, and David H. Kohn "Polarized Raman spectroscopy: application to bone biomechanics", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530W (8 February 2008); https://doi.org/10.1117/12.763520

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