Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage

Karen A. Esmonde-White; Gurjit S. Mandair; Farhang Raaii; Blake J. Roessler; Michael D. Morris

doi:10.1117/12.770360

22 February 2008 Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage

Karen A. Esmonde-White, Gurjit S. Mandair, Farhang Raaii, Blake J. Roessler, Michael D. Morris

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68530Y (2008) https://doi.org/10.1117/12.770360
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Human synovial fluid droplets were investigated using drop deposition in combination with Raman spectroscopy. Following informed consent, synovial fluid was obtained from forty human patients with various severities of knee pain and/or osteoarthritis at the time of knee arthroscopy or total joint replacement. Synovial fluid was aspirated from the knee joint of each patient and stored at -80°C until examination by near-infrared Raman spectroscopy. Synovial fluid aspirates from the knee joint of each patient were deposited onto a clean fused silica microscope slide and the droplet dried under ambient laboratory conditions. Each droplet was illuminated by a line-focused or a ring-focused 785 nm laser. As the droplet dries, biofluid components segregated based on solubility differences and a deposit that is spatially heterogeneous was made. Spectra taken from the droplet edges and center were dominated by protein bands and showed the presence of at least two protein moieties in the droplet. Band area and band height ratios (1410 cm^-1/1450 cm^-1) showed the greatest change between specimens from patients with mild/early osteoarthritis compared to those with severe/late stage osteoarthritis. The greatest differences were found in the center of the droplet, which contains more soluble protein components than the edges.

Citation Download Citation

Karen A. Esmonde-White, Gurjit S. Mandair, Farhang Raaii, Blake J. Roessler, and Michael D. Morris "Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530Y (22 February 2008); https://doi.org/10.1117/12.770360

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