The mechanism of PDT-induced electrical blockade: the measurement of intracellular Ca 2+ concentration changes in cardiac myocytes

A. Ito; S. Hosokawa; S. Hakomori; S. Miyoshi; K. Soejima; T. Arai

doi:10.1117/12.762058

20 February 2008 The mechanism of PDT-induced electrical blockade: the measurement of intracellular Ca²⁺ concentration changes in cardiac myocytes

A. Ito, S. Hosokawa, S. Hakomori, S. Miyoshi, K. Soejima, T. Arai

Author Affiliations +

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 68540M (2008) https://doi.org/10.1117/12.762058
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We propose the application of early state photodynamic therapy (PDT) to treatment of atrial fibrillation, which is a kind of arrhythmia characterized by irregular rapid beating of heart. We had demonstrated that our PDT can block the propagation of electrical excitation in cardiac myocytes. However, the mechanism of the PDT-induced electrical blockade was not clear. In order to clarify this mechanism, changes in intracellular Ca²⁺ concentration during the PDT with Talaporfin sodium (water soluble photosensitizer) were measured by fluorescence Ca²⁺ indicator, fluo-4 AM. The PDT led to the rapid increase of intracellular Ca²⁺ concentration and the changes in cell shapes. These results indicated that extracellular Ca²⁺ flowed into the cells mediated by cell membrane. Moreover, we found bubble generation in the cells after the PDT. In conclusion, the PDT-induced electrical blockade in myocytes can be caused by cell death following the bubble generation, which is accompanied by the increase in intracellular Ca²⁺ concentration due to the cell membrane malfunction with the PDT.

Citation Download Citation

A. Ito, S. Hosokawa, S. Hakomori, S. Miyoshi, K. Soejima, and T. Arai "The mechanism of PDT-induced electrical blockade: the measurement of intracellular Ca²⁺ concentration changes in cardiac myocytes", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 68540M (20 February 2008); https://doi.org/10.1117/12.762058

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