An in vitro corneal model with a laser damage threshold at 2 &#956;m that is similar to that in the rabbit

Michael S. Foltz; Michael L. Denton; Kurt J. Schuster; Larry E. Estlack; Semih S. Kumru

doi:10.1117/12.763543

20 February 2008 An in vitro corneal model with a laser damage threshold at 2 μm that is similar to that in the rabbit

Michael S. Foltz, Michael L. Denton, Kurt J. Schuster, Larry E. Estlack, Semih S. Kumru

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 685415 (2008) https://doi.org/10.1117/12.763543
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Corneal organotypic cultures were generated as per existing methods, which included growth on polycarbonate inserts and air-lifting for one week. The corneal simulant cultures were exposed, with real-time IR imaging, to the 2-μm wavelength output of a thulium fiber laser with 4 mm beam diameter for 0.25 seconds in a thermally controlled environment and then assayed for damage. The in vitro threshold (ED₅₀ value of 12.5 W/cm²) and peak temperature (74.5 °C) at threshold irradiance are compared with rabbit corneal data in the literature.

Citation Download Citation

Michael S. Foltz, Michael L. Denton, Kurt J. Schuster, Larry E. Estlack, and Semih S. Kumru "An in vitro corneal model with a laser damage threshold at 2 μm that is similar to that in the rabbit", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685415 (20 February 2008); https://doi.org/10.1117/12.763543

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