20 February 2008 An in vitro corneal model with a laser damage threshold at 2 μm that is similar to that in the rabbit
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Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 685415 (2008); doi: 10.1117/12.763543
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
Corneal organotypic cultures were generated as per existing methods, which included growth on polycarbonate inserts and air-lifting for one week. The corneal simulant cultures were exposed, with real-time IR imaging, to the 2-μm wavelength output of a thulium fiber laser with 4 mm beam diameter for 0.25 seconds in a thermally controlled environment and then assayed for damage. The in vitro threshold (ED50 value of 12.5 W/cm2) and peak temperature (74.5 °C) at threshold irradiance are compared with rabbit corneal data in the literature.
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Michael S. Foltz, Michael L. Denton, Kurt J. Schuster, Larry E. Estlack, Semih S. Kumru, "An in vitro corneal model with a laser damage threshold at 2 μm that is similar to that in the rabbit", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685415 (20 February 2008); doi: 10.1117/12.763543; http://dx.doi.org/10.1117/12.763543
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KEYWORDS
In vitro testing

In vivo imaging

Cameras

Laser damage threshold

Infrared cameras

Thermal modeling

Thermography

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