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20 February 2008Enhancement of light in tissue using hyper-osmotic agents
Raiyan T. Zaman,1 Bo Chen,1 Ashwin B. Parthasarathy,1 Arnold D. Estrada Jr.,1 Ardien Ponticorvo,1 Henry G. Rylander III,1 Andrew K. Dunn,1 Ashley J. Welch1
Optical changes in skin blood flow due to the presence of glycerol were measured from a two-dimensional map of blood
flow in skin blood vessels with a dynamic imaging technique using laser speckle. In this study a dorsal skin-flap window
was implanted on the hamster skin with and without a hyper-osmotic agent i.e. glycerol. The hyper-osmotic drug was
delivered to the skin through the open dermal end of the window model. A two-dimensional map of blood flow in skin
blood vessels were obtained with very high spatial and temporal resolution by imaging the speckle pattern with a CCD
camera. Preliminary studies demonstrated that hyper-osmotic agents such as glycerol not only make tissue temporarily
translucent, but also reduce blood flow. The blood perfusion was measured every 3 minutes up to 36-60 minutes after
diffusion of anhydrous glycerol. Small capillaries blood flow reduced significantly within 3-9 minutes. Perfusion rate in
lager blood vessels i.e. all arteries and some veins decreased (speckle contrasts increased from 0.0115 to 0.384) over
time. However, the blood flow in some veins reduced significantly in 36 minutes. After 24 hours the blood perfusion
further reduced in capillaries. However, the blood flow increased in larger blood vessels in 24 hours compared to an hour
after application of glycerol. For further investigation the speckle contrast measurement were verified with color
Doppler optical coherence tomography.
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Raiyan T. Zaman, Bo Chen, Ashwin B. Parthasarathy, Arnold D. Estrada Jr., Ardien Ponticorvo, Henry G. Rylander III, Andrew K. Dunn, Ashley J. Welch, "Enhancement of light in tissue using hyper-osmotic agents," Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 68541F (20 February 2008); https://doi.org/10.1117/12.763241