Morphometric effects of different energy densities of diode laser on adipose tissue in rats

Halina C. Senhorinho; Gerson L. Bichinho; Percy Nohama; Munir A. Gariba

doi:10.1117/12.760965

20 February 2008 Morphometric effects of different energy densities of diode laser on adipose tissue in rats

Halina C. Senhorinho, Gerson L. Bichinho, Percy Nohama, Munir A. Gariba

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 68541J (2008) https://doi.org/10.1117/12.760965
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

The aim of this study consisted in evaluating the influence of low power laser on the morfometry of white adipose cells in rats. The sample consisted of 20 adult female rats, from Wistar strain, randomized in four groups. All groups were submitted to tricotomy of the dorsal thoracic area and the first three groups were exposed to a InGaAlP laser (660 nm wavelength) with fluencies of 2, 8 and 16 J/cm² for groups L1, L2 and L3, respectively. L4 was the control. The groups were submitted to the protocols three times a week, for three weeks. The irradiated tissue was excised and submitted to histological treatment by HE for optical microscopy and the Kruskal-Wallis test was used for the statistical analysis. The average morphometry, in number of pixels, was: 3741 ± 704, 3762 ±947, 3737 ±1076 and 4619 ±781 for L1, L2, L3 and L4, respectively. There was no significant difference among groups L1 to L3 for the variable tested. From these results, it can be concluded that the application of low power laser - under the conditions proposed and for the fluency values chosen - influence the morphometry of white adipose cells in rats in the same manner, producing similar results.

Citation Download Citation

Halina C. Senhorinho, Gerson L. Bichinho, Percy Nohama, and Munir A. Gariba "Morphometric effects of different energy densities of diode laser on adipose tissue in rats", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 68541J (20 February 2008); https://doi.org/10.1117/12.760965

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