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28 February 2008 Multi-spectral photo-acoustic molecular tomography resolves fluorochrome distribution with high resolution and sensitivity in small animals
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Abstract
Current non-invasive imaging methods of fluorescent molecular probes in the visible and near-infrared suffer from low spatial resolution as a result of rapid light diffusion in biological tissues. We show that three-dimensional distribution of fluorochromes deep in small animals can be resolved with below 25 femtomole sensitivity and 150 microns spatial resolution by means of multi-spectral photoacoustic molecular tomography. The low sensitivity limit of the method is enabled by using the highly resonant absorption spectrum of a commonly used near-infrared fluorescent molecular probe Alexa Fluor® 750 in order to acquire differential images at multiple wavelengths with tomographic topology suitable for whole-body small animal imaging.
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Daniel Razansky, Claudio Vinegoni, and Vasilis Ntziachristos "Multi-spectral photo-acoustic molecular tomography resolves fluorochrome distribution with high resolution and sensitivity in small animals", Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 685613 (28 February 2008); https://doi.org/10.1117/12.764155
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