18 February 2008 PDT-apoptotic tumor cells induce macrophage immune response
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Abstract
Photodynamic therapy (PDT) functions as a cancer therapy through two major cell death mechanisms: apoptosis and necrosis. Immunological responses induced by PDT has been mainly associated with necrosis while apoptosis associated immune responses have not fully investigated. Heat shock proteins (HSPs) play an important role in regulating immune responses. In present study, we studied whether apoptotic tumor cells could induce immune response and how the HSP70 regulates immune response. The endocytosis of tumor cells by the activated macrophages was observed at single cell level by LSM. The TNF-α release of macrophages induced by co-incubated with PDT-apoptotic tumor cells was detected by ELISA. We found that apoptotic tumor cells treated by PDT could activate the macrophages, and the immune effect decreased evidently when HSP70 was blocked. These findings not only show that apoptosis can induce immunological responses, but also show HSP70 may serves as a danger signal for immune cells and induce immune responses to regulate the efficacy of PDT.
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Fei-fan Zhou, Da Xing, Wei R. Chen, "PDT-apoptotic tumor cells induce macrophage immune response", Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570C (18 February 2008); doi: 10.1117/12.759703; https://doi.org/10.1117/12.759703
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