18 February 2008 Spatial and temporal changes in Bax subcellular localization during NPe6-PDT-induced apoptosis
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Abstract
Photodynamic therapy (PDT) employing photosensiter N-aspartyl chlorin e6 (NPe6) can induce lysosome disruption and initiate the intrinsic apoptotic pathway. Bax, a member of the Bcl-2 family of proteins, is an essential regulator of apoptosis. Bax is normally found in the cytosol of healthy cells, and translocates to mitochondria in response to many apoptotic stimuli. In this study, using real-time single-cell analysis, we have investigated the kinetics of Bax distribution during NPe6-induced apoptosis in ASTC-a-1 cells. In order to monitor Bax subcellular distribution, cells were stained with GFP-Bax and Mito Tracker Red. The results show that Bax redistribution occurred at about 170 min after treated with NPe6-PDT, and then sequestered into clusters associated with the mitochondira within 30 min. Our data clearly showed the spatial and temporal changes in Bax distribution in living cells during NPe6-induced apoptosis.
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Lei Liu, Da Xing, Wei R. Chen, Qingling Wan, Feifan Zhou, "Spatial and temporal changes in Bax subcellular localization during NPe6-PDT-induced apoptosis", Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570D (18 February 2008); doi: 10.1117/12.762026; https://doi.org/10.1117/12.762026
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