Fluorescence imaging analysis of taxol-induced ASTC-a-1 cell death with cell swelling and cytoplasmic vacuolization

Tong-sheng Chen; Lei Sun; Longxiang Wang; Huiying Wang

doi:10.1117/12.761485

18 February 2008 Fluorescence imaging analysis of taxol-induced ASTC-a-1 cell death with cell swelling and cytoplasmic vacuolization

Tong-sheng Chen, Lei Sun, Longxiang Wang, Huiying Wang

Author Affiliations +

Proceedings Volume 6857, Biophotonics and Immune Responses III; 68570L (2008) https://doi.org/10.1117/12.761485
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Taxol (Paclitaxel), an isolated component from the bark of the Pacific yew Taxus brevifolia, exhibits a broad spectrum of clinical activity against human cancers. Taxol can promote microtubule (MT) assembly, inhibit depolymerization, and change MT dynamics, resulting in disruption of the normal reorganization of the microtubule network required for mitosis and cell proliferation. However, the molecular mechanism of taxol-induced cell death is still unclear. In this report, CCK-8 was used to assay the inhibition of taxol on the human lung adenocarcinoma (ASTC-a-1) cells viability, confocal fluorescence microscope was used to monitor the morphology changes of cells with taxol treatment. We for the first time describe the characteristics of taxol-induced cells swelling, cytoplasmic vacuolization and cell death. Taxol induced swelling, cytoplasmatic vacuolization and cell death without cell shrinkage and membrane rupture. These features differ from those of apoptosis and resemble the paraptosis, a novel nonapoptotic PCD.

Citation Download Citation

Tong-sheng Chen, Lei Sun, Longxiang Wang, and Huiying Wang "Fluorescence imaging analysis of taxol-induced ASTC-a-1 cell death with cell swelling and cytoplasmic vacuolization", Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570L (18 February 2008); https://doi.org/10.1117/12.761485

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