11 February 2008 Application of polarization OCT in tissue engineering
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Abstract
For tissue engineering of load-bearing tissues, such as bone, tendon, cartilage, and cornea, it is critical to generate a highly organized extracellular matrix. The major component of the matrix in these tissues is collagen, which usually forms a highly hierarchical structure with increasing scale from fibril to fiber bundles. These bundles are ordered into a 3D network to withstand forces such as tensile, compressive or shear. To induce the formation of organized matrix and create a mimic body environment for tissue engineering, in particular, tendon tissue engineering, we have fabricated scaffolds with features to support the formation of uniaxially orientated collagen bundles. In addition, mechanical stimuli were applied to stimulate tissue formation and matrix organization. In parallel, we seek a nondestructive tool to monitor the changes within the constructs in response to these external stimulations. Polarizationsensitive optical coherence tomography (PSOCT) is a non-destructive technique that provides functional imaging, and possesses the ability to assess in depth the organization of tissue. In this way, an engineered tissue construct can be monitored on-line, and correlated with the application of different stimuli by PSOCT. We have constructed a PSOCT using a superluminescent diode (FWHM 52nm) in this study and produced two types of tendon constructs. The matrix structural evolution under different mechanical stimulation has been evaluated by the PSOCT. The results in this study demonstrate that PSOCT was a powerful tool enabling us to monitor non-destructively and real time the progressive changes in matrix organization and assess the impact of various stimuli on tissue orientation and growth.
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Ying Yang, Ying Yang, Mark Ahearne, Mark Ahearne, Pierre O. Bagnaninchi, Pierre O. Bagnaninchi, Bin Hu, Bin Hu, Karen Hampson, Karen Hampson, Alicia J. El Haj, Alicia J. El Haj, } "Application of polarization OCT in tissue engineering", Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 68580K (11 February 2008); doi: 10.1117/12.762958; https://doi.org/10.1117/12.762958
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