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7 February 2008Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy
Julia G. Lyubovitsky,1 Xiaoman Xu,2 Chung-ho Sun,3 Bogi Andersen,2 Tatiana B. Krasieva,3 Bruce J. Tromberg3,2 1Univ. of California, Riverside (United States) 2Univ. of California, Irvine (United States) 3Beckman Laser Institute, Univ. of California, Irvine (United States)
Employing a reflectance multi-photon microscopy (MPM) technique, we developed novel method to
quantitatively study the three-dimensional assembly of structural proteins within bulk of dermal
ECMs. Using a structurally simplified model of skin with enzymatically dissected epidermis, we find
that low resolution MPM clearly discriminates between normal and pathological dermis. High-resolution
images revealed that the backscattered MPM signals are affected by the assembly of collagen fibrils and fibers within this system. Exposure of tissues to high concentrations of potentially denaturing chemicals also resulted in the reduction of SHG signals from structural
proteins which coincided with the appearance of aggregated fluorescent structures.
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Julia G. Lyubovitsky, Xiaoman Xu, Chung-ho Sun, Bogi Andersen, Tatiana B. Krasieva, Bruce J. Tromberg, "Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy," Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685902 (7 February 2008);