5 March 2008 The study of optimal condition of SPIO labeling human lung adenocarcinoma cell line (SPC-A-1)
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Abstract
Propose: To study the optimal concentration and time of incubation of human lung adenocarcinoma cell line (SPC-A-1) labeled with superparamagnetic iron oxide (SPIO) particles in vitro. Methods: Human lung adenocarcinoma cell line (SPC-A-1) was cultured with different concenration of SPIO and different time of incubation (labeled with media containing Fe-PLL: 25μg /mL, 100μg /mL, and 200 μg /mL, and for 30min, 90min, 180min. The phagocytosis of the cells was observed by laser scanning confocal microscopy (LSCM) to determine particle uptake and their distribution in cells. Results: Human lung adenocarcinoma cells(SPC-A-1) have taken up a large amount of SPIO particles within the first 3h. Conclusion: In this study, the concentration of iron with 25μg/ml SPIO and time of incubation for 30min is the optimal condition for labeling the SPC-A-1 with SPIO.
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Ming-xi Yu, Wen-li Chen, Quan Zhou, Da Xing, Yong-hong Tang, "The study of optimal condition of SPIO labeling human lung adenocarcinoma cell line (SPC-A-1)", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68591O (5 March 2008); doi: 10.1117/12.762032; https://doi.org/10.1117/12.762032
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