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12 March 2008 Coherent interaction of optical second harmonic generation in collagen fibrils
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We present the first experimental comparison between optical second harmonic generation images and atomic force microscope images in a matrix of nano-scaled collagen fibrils. Substantial variation of forward and backward propagated second harmonic generation radiation is observed in a single collagen fibril and is nicely correlated with the accurately determined thickness from an atomic force microscope. Contradicting to conventional nonlinear optical theory, our result indicates a linear relationship between fibril thickness and forward / backward second harmonic generation ratio. This is the first demonstration of estimating fibril thickness with nanometer precision by a noninvasive optical method.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Shi-Wei Chu, Shih-Peng Tai, Ming-Che Chan, Chi-Kuang Sun, I-Ching Hsiao, Chi-Hung Lin, Yung-Chih Chen, and Bai-Ling Lin "Coherent interaction of optical second harmonic generation in collagen fibrils", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686004 (12 March 2008);

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