Polarization dependant in vivo second harmonic generation imaging of Caenorhabditis elegans vulval, pharynx, and body wall muscles

Sotiris Psilodimitrakopoulos; Susana Santos; Ivan Amat-Roldan; Manoj Mathew; Anisha Thayil K. N.; David Artigas; Pablo Loza-Alvarez

doi:10.1117/12.763270

15 February 2008 Polarization dependant in vivo second harmonic generation imaging of Caenorhabditis elegans vulval, pharynx, and body wall muscles

Sotiris Psilodimitrakopoulos, Susana Santos, Ivan Amat-Roldan, Manoj Mathew, Anisha Thayil K. N., David Artigas, Pablo Loza-Alvarez

Author Affiliations +

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 686008 (2008) https://doi.org/10.1117/12.763270
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Second harmonic generation (SHG) imaging has emerged in recent years as an important laboratory imaging technique since it can provide unique structural information with submicron resolution. It enjoys the benefits of non-invasive interaction establishing this imaging modality as ideal for in vivo investigation of tissue architectures. In this study we present, polarization dependant high resolution SHG images of Caenorhabditis elegans muscles in vivo. We imaged a variety of muscular structures such as body walls, pharynx and vulva. By fitting the experimental data into a cylindrical symmetry spatial model we mapped the corresponding signal distribution of the χ⁽²⁾ tensor and identified its main axis orientation for different sarcomeres of the earth worm. The cylindrical symmetry was considered to arise from the thick filaments architecture of the inside active volume. Moreover, our theoretical analysis allowed calculating the mean orientation of harmonophores (myosin helical pitch). Ultimately, we recorded and analysed vulvae muscle dynamics, where SHG signal decreased during in vivo contraction.

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Sotiris Psilodimitrakopoulos, Susana Santos, Ivan Amat-Roldan, Manoj Mathew, Anisha Thayil K. N., David Artigas, and Pablo Loza-Alvarez "Polarization dependant in vivo second harmonic generation imaging of Caenorhabditis elegans vulval, pharynx, and body wall muscles", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686008 (15 February 2008); https://doi.org/10.1117/12.763270

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