Combined chi (3) microscopy of the peripheral nervous tissue

Christian P. Pfeffer; Martin Vogel; Bjorn R. Olsen; Feruz Ganikhanov

doi:10.1117/12.763743

21 February 2008 Combined chi(3) microscopy of the peripheral nervous tissue

Christian P. Pfeffer, Martin Vogel, Bjorn R. Olsen, Feruz Ganikhanov

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 686010 (2008) https://doi.org/10.1117/12.763743
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We report an application of the combined third order microscopy techniques to reveal structure and morphology of the peripheral nerve in mice. The resonant Coherent Anti-Stokes Raman Scattering (CARS) and third harmonic generation (THG) techniques have been applied to visualize structure of the myelinated peripheral axon. While CARS was quite efficient in selective imaging of the cladding layer via characteristic Raman active vibrations of dense lipid structures constituting the layers, the THG microscopy helped to clearly reveal the degree of optical and nonlinear optical inhomogeneity of the axon core (that may have further important implications).

Citation Download Citation

Christian P. Pfeffer, Martin Vogel, Bjorn R. Olsen, and Feruz Ganikhanov "Combined chi(3) microscopy of the peripheral nervous tissue", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686010 (21 February 2008); https://doi.org/10.1117/12.763743

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