15 February 2008 Serotonin: multiphoton imaging and relevant spectral data
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Coupling three-photon microscopy with automated stage movement can now produce a live high resolution map of the neurotransmitter serotonin in a single cross section of the whole rat brain. Accurate quantification of these serotonin images demands appropriate spectral filtering. This requires one to consider that the spectral characteristics of serotonin show a remarkable variation as it non-covalently associates with different molecules, as we discuss here. Also it is known that serotonin emission changes when it forms a covalent adduct with para-formaldehyde. This provides a potential route for producing a whole brain serotonin map using multiphoton microscopy in a fixed rat brain. Here we take the initial step showing that multiphoton microscopy of this adduct can quantitatively image chemically induced changes in serotonin distribution.
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S. K. Kaushalya, S. K. Kaushalya, Suman Nag, Suman Nag, J. Balaji, J. Balaji, S. Maiti, S. Maiti, } "Serotonin: multiphoton imaging and relevant spectral data", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601C (15 February 2008); doi: 10.1117/12.763020; https://doi.org/10.1117/12.763020

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