15 February 2008 SLIM for multispectral FRET imaging
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Abstract
Spectral fluorescence lifetime imaging (SLIM) is an advanced imaging technique, which combines spectral with time resolved detection. Real spectral information is achieved by using a grating in front of a PML-array, which allows time-correlated single photon counting (TCSPC). Whereas spectrally resolved fluorescence imaging alone has a reasonable sensitivity, the specificity of fluorescence detection can be improved by considering the fluorescence lifetime. The various possibilities which SLIM offers to improve FRET (resonant energy transfer) will be discussed as well as successfully realized applications. These include FRET measurements for protein interactions, related to Alzheimer's disease. Special attention will be focused on molecules involved in the processing and trafficking of the amyloid precursor protein (APP), as trafficking proteins of the GGA family and β-secretase BACE). Taking into account also the lifetime of the acceptor could enhance reliability of the FRET result.
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A. Rück, A. Rück, F. Dolp, F. Dolp, R. Steiner, R. Steiner, C. Steinmetz, C. Steinmetz, B. von Einem, B. von Einem, C.A.F. von Arnim, C.A.F. von Arnim, } "SLIM for multispectral FRET imaging", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601F (15 February 2008); doi: 10.1117/12.761660; https://doi.org/10.1117/12.761660
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