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15 February 2008 FLIM and FCS measurements performed with a master oscillator fiber amplifier (MOFA) laser at 530 nm
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Upgrade kits towards time-resolved measurements for Confocal Microscopes allow new measurement modes like Fluorescence Lifetime Imaging (FLIM), time-resolved analysis of Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Resonance Energy Transfer (FRET). Microscope users would typically like to use the same excitation wavelength for time-resolved measurements as for steady-state measurements, because their fluorophores are designed for the CW-laser wavelengths usually provided with the system. Pulsed diode lasers, which are ideally used for these upgrade kits are, however, not available for every spectral region of interest. Especially for "green" excitation around 530 nm this is still a problem, as there are no direct emitting laser diodes available. We present a new picosecond pulsed laser system for 530 nm emission with variable repetition rate and pulse energy, which is ideally suited for time-resolved measurements using Time-Correlated Single Photon Counting (TCSPC), and demonstrate its integration into a confocal microscope as well as first results of FLIM and FCS measurements.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Felix Koberling, Martin Langkopf, Dietmar Klemme, Andreas Bülter, Volker Buschmann, Kristian Lauritsen, and Rainer Erdmann "FLIM and FCS measurements performed with a master oscillator fiber amplifier (MOFA) laser at 530 nm", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601N (15 February 2008); doi: 10.1117/12.761229;

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