Two-photon laser scanning microscopy with ultrabroad bandwidth 110 nm FWHM femtosecond pulses

Peng Xi; Lindsay R. Weisel; Yair Andegeko; Vadim V. Lovozoy; Marcos Dantus

doi:10.1117/12.763698

15 February 2008 Two-photon laser scanning microscopy with ultrabroad bandwidth 110 nm FWHM femtosecond pulses

Peng Xi, Lindsay R. Weisel, Yair Andegeko, Vadim V. Lovozoy, Marcos Dantus

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601U (2008) https://doi.org/10.1117/12.763698
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Shorter pulses, in theory, should be favorable in nonlinear microscopy and yield stronger signals. However, shorter pulses are much more prone to chromatic dispersion when passing through the microscope objective, which significantly broadens its pulse duration and cancels the expected signal gain. In this paper, multiphoton intrapulse interference phase scan (MIIPS) was used to compensate chromatic dispersion introduced by the 1.45 NA objective. The results show that with MIIPS compensation, the increased signal is realized. We also find that third and higher order dispersion compensation, which cannot be corrected by prism pairs, is responsible for an additional factor of 4.7 signal gain.

Citation Download Citation

Peng Xi, Lindsay R. Weisel, Yair Andegeko, Vadim V. Lovozoy, and Marcos Dantus "Two-photon laser scanning microscopy with ultrabroad bandwidth 110 nm FWHM femtosecond pulses", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601U (15 February 2008); https://doi.org/10.1117/12.763698

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