Two-photon imaging of stem cells

A. Uchugonova; E. Gorjup; I. Riemann; D. Sauer; K. König

doi:10.1117/12.762734

15 February 2008 Two-photon imaging of stem cells

A. Uchugonova, E. Gorjup, I. Riemann, D. Sauer, K. König

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601W (2008) https://doi.org/10.1117/12.762734
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

A variety of human and animal stem cells (rat and human adult pancreatic stem cells, salivary gland stem cells, dental pulpa stem cells) have been investigated by femtosecond laser 5D two-photon microscopy. Autofluorescence and second harmonic generation have been imaged with submicron spatial resolution, 270 ps temporal resolution, and 10 nm spectral resolution. In particular, NADH and flavoprotein fluorescence was detected in stem cells. Major emission peaks at 460nm and 530nm with typical mean fluorescence lifetimes of 1.8 ns and 2.0 ns, respectively, were measured using time-correlated single photon counting and spectral imaging. Differentiated stem cells produced the extracellular matrix protein collagen which was detected by SHG signals at 435 nm.

Citation Download Citation

A. Uchugonova, E. Gorjup, I. Riemann, D. Sauer, and K. König "Two-photon imaging of stem cells", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601W (15 February 2008); https://doi.org/10.1117/12.762734

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