15 February 2008 Two-photon fluorescence imaging and femtosecond laser microsurgery to study drosophila dorsal closure
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Abstract
Dorsal closure is a key morphogenic process that occurs at the last stages of Drosophila melanogaster embryogenesis. It involves a well coordinated rearrangement and movement of tissues that resemble epithelial wound healing in mammals. The cell dynamics and intracellular signaling pathways that accompany hole closure are expected to be similar during would healing providing a model system to study epithelial healing. Here we demonstrate the use of two-photon fluorescence microscope together with femtosecond laser ablation to examine the epithelial wound healing during embryonic dorsal closure. By using tightly focused NIR femtosecond pulses of subnanojoule energy we are able to produce highly confined microsurgery on the epithelial cells of a developing embryo. We observed that drosophila epidermis heals from the laser wounds with increased activity of actin near the wound edges.
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Anisha Thayil K. N., Andrea Pereira, Manoj Mathew, David Artigas, Enrique Martín Blanco, Pablo Loza-Alvarez, "Two-photon fluorescence imaging and femtosecond laser microsurgery to study drosophila dorsal closure", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601Z (15 February 2008); doi: 10.1117/12.762741; https://doi.org/10.1117/12.762741
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