Axial resolution for two-photon wide-field illumination microscopy and microfabrication

Daekeun Kim; Peter So

doi:10.1117/12.784748

15 February 2008 Axial resolution for two-photon wide-field illumination microscopy and microfabrication

Daekeun Kim; Peter So

Author Affiliations +

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 686028 (2008) https://doi.org/10.1117/12.784748
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

For the last two decades, multiphoton excitation microscopy/microfabrication based on laser scanning/writing techniques has been popular in the life science as well as photonics. Due to the slow scanning/writing nature, these applications are very limited to the production of prototypes, although its submicron optical resolution and intrinsic 3D optical sectioning capability are very attractive for creating 3D structures. In this proceeding, we introduced multiphoton excitation microscopy and microfabrication based on wide-field illumination. We derived mathematical model for wide-field illumination in the microscopy and microfabrication, and identified the design parameters that affect axial resolution for the proposed system. The future work of developing optical model combined with photopolymerization is also discussed.

Citation Download Citation

Daekeun Kim, Daekeun Kim, Peter So, Peter So, } "Axial resolution for two-photon wide-field illumination microscopy and microfabrication", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686028 (15 February 2008); doi: 10.1117/12.784748; https://doi.org/10.1117/12.784748

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