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12 February 2008Aberration free refocusing for high numerical aperture microscopy
In this paper, we present a novel technique that permits sectioning microscopes to refocus and acquire images
from a large range of specimen depths without introducing movements near the specimen. In contrast to other
such remote focusing methods, this technique avoids systematic aberrations that degrade image quality when
imaging planes away from the optimal focal plane. Furthermore, the specific geometry that is employed in this
work enables refocusing to be carried out at high speed and hence permits, for the first time, a number of
dynamical biological processes to be observed. Although this technique can be applied to any optical imaging
system, it is particularly suited to the case of high numerical aperture microscope systems. To demonstrate
this we present results from two prototype systems built in our laboratory based on a slit scanning confocal
fluorescence microscope and a two photon fluorescence microscope.
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Edward Botcherby, Rimas Juškaitis, Martin Booth, Tony Wilson, "Aberration free refocusing for high numerical aperture microscopy," Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686110 (12 February 2008); https://doi.org/10.1117/12.771419