21 February 2008 Fluorescence microscope using total internal reflection
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Abstract
In this experimental investigation, we suggest a new fluorescence microscope and method using a total internal reflection fluorescence. Unlike the conventional a total internal reflection fluorescence scheme, it uses fluorescence obtained due to the excitation due to the excitation of micro-objects occurring over the entire thickness of a sample, not a region in which an evanescent field extends. By the proposed scheme, the observation of the fluorescence of a biological micochip is possible without the installation of optical filters on excitation and reception light paths. It is possible to observe a small amount of fluorescent dye due to the reduction of the occurrence of optical noises and to simultaneously observe fluorescence images emitted from various fluorescent dyes without optical filters, so that its construction become simple and the size decreases.
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Soo Jin Bae, Soo Jin Bae, Seung Yup Lee, Seung Yup Lee, Uk Kang, Uk Kang, } "Fluorescence microscope using total internal reflection", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686210 (21 February 2008); doi: 10.1117/12.764347; https://doi.org/10.1117/12.764347
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