Suppressing nonspecific adsorption of proteins on the single-molecular level

Nicole Marmé; Haemi Lee; Achim Friedrich; Chong-Woo Park; Julie Fiore; David J. Nesbitt; Jens-Peter Knemeyer

doi:10.1117/12.763014

21 February 2008 Suppressing nonspecific adsorption of proteins on the single-molecular level

Nicole Marmé, Haemi Lee, Achim Friedrich, Chong-Woo Park, Julie Fiore, David J. Nesbitt, Jens-Peter Knemeyer

Author Affiliations +

Proceedings Volume 6862, Single Molecule Spectroscopy and Imaging; 686212 (2008) https://doi.org/10.1117/12.763014
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Avoiding nonspecific surface adsorption is a crucial and often challenging issue in many single-molecule studies and analytical applications. In this work, we investigated glass surfaces coated with cross-linking star-shaped polyethylene glycol (4-arm PEG) and demonstrated that this coating can be used for effective suppression of nonspecific protein binding, such as streptavidin. Single-molecule fluorescence images show that only a few molecules remain nonspecifically bound to surfaces treated with protein after sufficient rinsing, i.e. less than to a state-of-the-art BSA coating. Furthermore, different applications for star-shaped PEG-passivated surfaces are shown.

Citation Download Citation

Nicole Marmé, Haemi Lee, Achim Friedrich, Chong-Woo Park, Julie Fiore, David J. Nesbitt, and Jens-Peter Knemeyer "Suppressing nonspecific adsorption of proteins on the single-molecular level", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686212 (21 February 2008); https://doi.org/10.1117/12.763014

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