21 February 2008 Suppressing nonspecific adsorption of proteins on the single-molecular level
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Avoiding nonspecific surface adsorption is a crucial and often challenging issue in many single-molecule studies and analytical applications. In this work, we investigated glass surfaces coated with cross-linking star-shaped polyethylene glycol (4-arm PEG) and demonstrated that this coating can be used for effective suppression of nonspecific protein binding, such as streptavidin. Single-molecule fluorescence images show that only a few molecules remain nonspecifically bound to surfaces treated with protein after sufficient rinsing, i.e. less than to a state-of-the-art BSA coating. Furthermore, different applications for star-shaped PEG-passivated surfaces are shown.
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Nicole Marmé, Nicole Marmé, Haemi Lee, Haemi Lee, Achim Friedrich, Achim Friedrich, Chong-Woo Park, Chong-Woo Park, Julie Fiore, Julie Fiore, David J. Nesbitt, David J. Nesbitt, Jens-Peter Knemeyer, Jens-Peter Knemeyer, } "Suppressing nonspecific adsorption of proteins on the single-molecular level", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686212 (21 February 2008); doi: 10.1117/12.763014; https://doi.org/10.1117/12.763014

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