Combining AFM and FRET for studies at the cellular level

Kenith E. Meissner; Zhe Sun; Bhavik Nathwani; Clark Needham; Richard E. Beckham; William N. Everett; Xudong Fan; Gerard L. Cote; Gerald A. Meininger

doi:10.1117/12.769175

22 February 2008 Combining AFM and FRET for studies at the cellular level

Kenith E. Meissner; Zhe Sun; Bhavik Nathwani; Clark Needham; Richard E. Beckham; William N. Everett; Xudong Fan; Gerard L. Cote; Gerald A. Meininger

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Proceedings Volume 6863, Optical Diagnostics and Sensing VIII; 686302 (2008) https://doi.org/10.1117/12.769175
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We report on the development of a Forster resonance energy transfer (FRET) enabled atomic force microscope (AFM) for the study of biomechanics and mechanobiology at the cellular level. The hybrid microscopy system combines the spatial resolution and control of the AFM with the nanoscale sensing capabilities of FRET to enable simultaneous detection of cell mechanical responses and correlation of those responses with cellular biochemistry. Here, we show FRET signal from donor-coated microspheres, that are attached to an AFM cantilever, to acceptor-labeled integrins in a fixed cell system. Additionally, we demonstrate and discuss the attachment of quantum dots to silica microspheres as the FRET donor.

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Kenith E. Meissner, Kenith E. Meissner, Zhe Sun, Zhe Sun, Bhavik Nathwani, Bhavik Nathwani, Clark Needham, Clark Needham, Richard E. Beckham, Richard E. Beckham, William N. Everett, William N. Everett, Xudong Fan, Xudong Fan, Gerard L. Cote, Gerard L. Cote, Gerald A. Meininger, Gerald A. Meininger, } "Combining AFM and FRET for studies at the cellular level", Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 686302 (22 February 2008); doi: 10.1117/12.769175; https://doi.org/10.1117/12.769175

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