Over the last two decades, optical polarimetry method has been applied for glucose concentration monitoring in biological media such as aqueous humor and cell culture media as a non-invasive alternative method. Compared to glucose analyzer and electronic analyte sensor, the advantages of polaimetry method are non-ionizing radiation to interrogate the sample, non-interference with the sample, needless of consumable reagents, use of readily available sources, and prospect of miniaturizing the optics. Commercial polarimeter is widely used to measure the concentration of chemical compounds with optical activity. However, this device was based on off-line measurement so that it needs sample extraction process to measure the concentration of sample. This process does not reflect the real-time status of sample concentration and sample contamination can be occurred during sample extraction when applied to cell culture process. In polarimetry method, the measurement sensitivity can be controlled by varying optical path length. However, in current polarimeter, the sample cell should be exchanged to vary the optical path length. This process is a time consuming and might cause sample contamination in cell culture process. Therefore, it is necessary to develop a new polarimetry method which can measure the real-time status of sample concentration without sample extraction. In this paper, we introduce a new polarimetry probe system which might be utilized to monitor glucose concentration during cell culture process. It was designed to have variable optical path lengths to control the optical rotation angle of polarized light. We describe the feasibility of the system and the preliminary results.