28 February 2008 Enlarging the linear response range of velocity with optimum imaging parameters and modified data processing in laser speckle imaging
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Abstract
Laser speckle imaging (LSI) technique is considered as a promising method of accessing cerebral blood flow (CBF) of animals for its high spatiotemporal resolution and simplicity. It is important in LSI that optimum imaging parameters and limited noises should be confirmed to promote the imaging precision. We investigated in this paper different factors which may affect the imaging results with a moving white plate model, and then proposed a method of enlarging the linear response range of velocity. Through experiment, we proposed in our LSI system the optimum imaging parameters, including the numerical aperture and magnification of microscopy, the integration time, the gain mode of CCD camera. The average intensity was found optimum at about 800 counts out of 4096 grey level, which permits the highest contrast in our experiment. To eliminate the influence of uneven illumination, a direct current weight of 27 counts was subtracted during data processing. The result indicated that the relationship between measured velocity and the real one remained linear with R2 equaling to 0.99 throughout the scale of 80 mm/s.
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Jianjun Qiu, Jianjun Qiu, Pengcheng Li, Pengcheng Li, Sergey S. Ul'yanov, Sergey S. Ul'yanov, Shaoqun Zeng, Shaoqun Zeng, Qingming Luo, Qingming Luo, } "Enlarging the linear response range of velocity with optimum imaging parameters and modified data processing in laser speckle imaging", Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 68630T (28 February 2008); doi: 10.1117/12.763355; https://doi.org/10.1117/12.763355
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