22 February 2008 Relationship between light scattering and absorption due to cytochrome c oxidase reduction during loss of tissue viability in brains of rats
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Abstract
We performed simultaneous measurement of light scattering and absorption due to reduction of cytochrome c oxidase as intrinsic optical signals that are related to morphological characteristics and energy metabolism, respectively, for rat brains after oxygen/glucose deprivation by saline infusion. To detect change in light scattering, we determined the wavelength that was the most insensitive to change in light absorption due to the reduction of cytochrome c oxidase on the basis of multiwavelength analysis of diffuse reflectance data set for each rat. Then the relationships between scattering signal and absorption signals related to the reductions of heme aa3 (605 nm) and CuA (830 nm) in cytochrome c oxidase were examined. Measurements showed that after starting saline infusion, the reduction of heme aa3 started first; thereafter triphasic, large scattering change occurred (200-300 s), during which the reduction of CuA started. Despite such complex behaviors of IOSs, almost linear correlations were seen between the scattering signal and the heme aa3-related absorption signal, while a relatively large animal-to-animal variation was observed in the correlation between the scattering signal and CuA-related absorption signal. Transmission electron microscopic observation revealed that dendritic swelling and mitochondrial deformation occurred in the cortical surface tissue after the triphasic scattering change. These results suggest that mitochondrial energy failure accompanies morphological alteration in the brain tissue and results in change in light scattering; light scattering will become an important indicator of tissue viability in brain.
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Satoko Kawauchi, Satoko Kawauchi, Shunichi Sato, Shunichi Sato, Hidetoshi Ooigawa, Hidetoshi Ooigawa, Hiroshi Nawashiro, Hiroshi Nawashiro, Miya Ishihara, Miya Ishihara, Makoto Kikuchi, Makoto Kikuchi, } "Relationship between light scattering and absorption due to cytochrome c oxidase reduction during loss of tissue viability in brains of rats", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640K (22 February 2008); doi: 10.1117/12.762811; https://doi.org/10.1117/12.762811
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