1 March 2008 Time-resolved optical spectroscopic quantification of red blood cell damage caused by cardiovascular devices
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Cardiovascular devices such as heart-lung machine generate un-physiological level of shear stress to damage red blood cells, leading to hemolysis. The diagnostic techniques of cell damages, however, have not yet been established. In this study, the time-resolved optical spectroscopy was applied to quantify red blood cell (RBC) damages caused by the extracorporeal circulation system. Experimentally, the fresh porcine blood was subjected to varying degrees of shear stress in the rotary blood pump, followed with measurement of the time-resolved transmission characteristics using the pico-second pulses at 651 nm. The propagated optical energy through the blood specimen was detected using a streak camera. The data were analyzed in terms of the mean cell volume (MCV) and mean cell hemoglobin concentration (MCHC) measured separately versus the energy and propagation time of the light pulses. The results showed that as the circulation time increased, the MCV increased with decrease in MCHC. It was speculated that the older RBCs with smaller size and fragile membrane properties had been selectively destroyed by the shear stress. The time-resolved optical spectroscopy is a useful technique in quantifying the RBCs' damages by measuring the energy and propagation time of the ultra-short light pulses through the blood.
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D. Sakota, R. Sakamoto, H. Sobajima, N. Yokoyama, Y. Yokoyama, S. Waguri, K. Ohuchi, S. Takatani, "Time-resolved optical spectroscopic quantification of red blood cell damage caused by cardiovascular devices", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 686410 (1 March 2008); doi: 10.1117/12.762607; https://doi.org/10.1117/12.762607

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